Figure 2.

Specc1l^cGT/ΔC510 compound mutants exhibit palatal shelf elevation delay. (A–G) Representative whole mount images (top row) and coronal H&E palate sections (bottom three rows) of control and Specc1l^cGT/ΔC510 embryos from E13.5 to E15.5. Compound mutants show edema, midline subepidermal blebbing (B and D; arrowheads) and occasional hemorrhaging (F and G; arrowheads) compared to control embryos. At E14.5, ~66% of mutant embryos had palatal shelves that had not elevated (D; arrows) compared to littermate controls (C; arrows). By E15.5, most mutants had closed palates (F), indicating that these mutants experience a delay in palatal shelf elevation. However, cleft palate can occur in mutant embryos with severe phenotypes (G; arrows).