Figure 8.

Immunofluorescence microscopy images obtained on SK28 human melanoma cell line grown on GONB MAPLE thin films without (A) or with (B) DAB BRAF inhibitor. SK28 malignant cells grown in standard conditions in the absence (C), or in the presence (D) of 0.5 nM DAB were used as controls of DAB activity. The comparison with SK23 melanoma cells that express wild type BRAF is presented in (E). Cells were stained with anti-phospho-ERK antibodies (red) and Phalloidin (green) for exhibiting MAPK pathway activation and the cellular cytoskeleton consisting of actin filaments, respectively. Nuclei were stained with Hoechst (blue). Scale bar = 50 μm.