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. 2019 Dec 12;142(4):0410031–04100312. doi: 10.1115/1.4044899

Fig. 3.

Fig. 3

NO was elevated in BAEC after 36 h in the laminar but not disturbed flow parallel plate flow chamber. (a, top) BAEC confocal microscopy images (20×) in varied flow regimes for 36 h showing NO (DAF-FM, green) and nuclei (bisbenzimide, blue). (a, bottom) NO only (DAF-FM, green) confocal microscopy images (20×). (b, left) DAF signal after thresholding to remove noise. (b, right) Total DAF-FM fluorescence intensity quantification for BAEC exposed to static or flow conditions for 36 h (122 images among 18 samples, across 4 independent experiments). *** indicates p < 0.0001 (LFG compared to all other conditions).