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. Author manuscript; available in PMC: 2020 Dec 19.
Published in final edited form as: Mol Cell. 2019 Oct 3;76(6):965–980.e12. doi: 10.1016/j.molcel.2019.08.030

Figure 2. H3.3K27M and H3.1K27M DIPG show discrete profiles of accessibility and oncohistone localization.

Figure 2.

A) Clustering of accessibility at enhancer elements in primary DIPG samples and normal pediatric pons. Data shown as PCA of Omni-ATAC signal. Colors indicate clusters (n = 9 primary human tissue samples; 7 DIPG, 2 non-malignant pediatric pons).

B) GREAT analysis of differentially accessible enhancer elements between H3.3K27M Group A (n = 3) and H3.1K27M DIPG (n = 2).

C) Representative Omni-ATAC track. X-axis represents genomic location and y-axis represents reads per million (RPM). Black bars indicate differential distal peaks.

D) Top shared motifs enriched over normal pons (H3.3K27M Group A, n = 3; H3.1K27M, n = 2; normal pons, n = 2). Ordered by average differential enrichment over normal pons.

E) Motifs with different signal between H3.3K27M and H3.1K27M DIPG.

F) Enrichment plots of WT H3.3 or H3K27M signal at H3K27Ac peaks in H3.3K27M (left) or H3.1K27M (right) patient-derived cultures. Y-axis shows reads per million (RPM) (n = 2).

G) Scatter plots of mean H3.3 or H3K27M enrichment at all H3.3 peaks in H3.3K27M (left) or H3.1K27M (right) patient-derived cultures (n = 2).

H) Representative genome tracks showing H3K27Ac, H3K27M, and H3.3 localization in SU-DIPG cultures. X-axis represents genomic location and y-axis represents RPM.

See Figure S3.