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. Author manuscript; available in PMC: 2020 Dec 19.
Published in final edited form as: Mol Cell. 2019 Oct 3;76(6):965–980.e12. doi: 10.1016/j.molcel.2019.08.030

Figure 4. p38 MAPK signaling facilitates cell growth in H3.1K27M DIPG.

Figure 4.

A) Differential H3K27Ac signal at p38 MAPK pathway gene MAPKAPK2. X-axis represents genomic location and y-axis represents RPM. Blue bars indicate differential enhancers and promoters activated in H3.1K27M over H3.3K27M Group A DIPG.

B) Experimental paradigm for GF sensitization of DIPG cultures and subsequent studies shown in (C) and (D).

C) Western blot in patient-derived DIPG cultures following treatment with B27 alone (−) or with GFs (+). PPIB shown as loading control.

D) Cell viability of patient-derived DIPG cultures following GF treatment with or without p38 MAPK inhibition (SB202190). Values for each culture are normalized to B27 only + DMSO treated cells (n = 3).

All pooled data shown as mean +/− s.d.; * adjusted p value < 0.1, ** adjusted p value < 0.01, *** adjusted p value < 0.001, **** adjusted p value < 0.0001.