A) PCA of H3K27me3 signal at all peaks in eOPCs following 14 days of H3K27M induction.
B) H3K27me3 change at CGIs in eOPCs following H3K27M induction for 14 days. X-axis represents mean log2(H3K27me3 enrichment) in control eOPCs (n = 3) and y-axis represents mean log2(H3K27me3 enrichment change) (n = 2 per H3K27M variant). Black dots represent CGIs with log2(H3K27me3 enrichment) > 1 in all control samples.
C) Correlation between mean H3.3 enrichment and H3K27me3 change at H3K27me3+ CGIs. X-axis represents mean log2(H3.3 enrichment) in control eOPCs and y-axis represents mean log2(H3K27me3 enrichment change).
D) Enrichment plots of ChIP-seq signal at high H3.3 (left) and low H3.3 (right) H3K27me3+ CGIs. Y-axis shows average ChIP-seq signal with H3.3 and EED shown as RPM (top) and H3K27me3 as reference-adjusted RPM (RRPM; bottom).
E) Comparison of mean ChIP-seq singal at H3K27me3+ CGIs in control eOPCs. Color scale indicates level of log2(H3.3 enrichment), x-axis shows log2(H3K27me3 enrichment), and y-axis shows log2(EED enrichment).
F) GREAT analysis on high H3.3 and low H3.3 H3K27me3+ CGIs.
See Figure S6.