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. 2020 Mar 25;13(3):dmm042218. doi: 10.1242/dmm.042218

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© 2020. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — Grhl2^−/− PA1 epithelium has mesenchymal characteristics. (A) Transverse sections through the E10.5 PA1 MDP stained by immunohistochemistry for E-cadherin (Ecad), Epcam or vimentin (Vim), or with H&E. Images are representative of four embryos. (B) As for panel A, except images are of coronal sections through the MXP. (C) Transverse sections through the MDP of E10.5 embryos stained for β-galactosidase (β-gal) activity. (D) Q-RT-PCR on E10.5 PA1 epithelium (epi) and mesenchyme (mes). Graph shows mean±s.d. n=6 embryos. Grhl2^+/+ and Grhl2^−/− epithelium were compared using Student's t-tests corrected for multiple comparisons with the Holm-Sidak method. *P<0.0001. Scale bars: 20 μm (A,B) and 40 μm (C).