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. 2020 Mar 25;13(3):dmm042218. doi: 10.1242/dmm.042218

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© 2020. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — GRHL2 maintains epithelial cellular identity via multiple direct target genes. Q-RT-PCR on E10.5 PA1 epithelium (epi) and mesenchyme (mes). (A) Epithelial and mesenchymal transcription factors. (B) Zeb1-repressing microRNAs and epithelial splicing regulators. (C) Isoform-specific Q-RT-PCR for Fgfr2, Cd44, Ctnnd1 and Enah. Grhl2^+/+ and Grhl2^−/− epithelium were compared using Student's t-tests corrected for multiple comparisons with the Holm-Sidak method. *P<0.05. n=5-6 embryos. (D) ChIP on E10.5 PA1 with Grhl2 antibody or normal rabbit immunoglobulin (IgG) antibody, followed by PCR with primers spanning predicted GRHL2 binding sites. Results are representative of three independent experiments. *P<0.0001 by two-way ANOVA with Sidak's multiple comparison test. Graphs show mean±s.d of quadruplicate Q-RT-PCRs.