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. 2020 Mar 24;9(3):bio048173. doi: 10.1242/bio.048173

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© 2020. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — MACF1 diminished interaction of EB1 with APC by influencing Src activation. (A) p[Y418] Src and APC levels in MC3T3-E1 cells are detected by western blot. Colocalization of EB1 with p[Y418] Src (B) and APC (C) on FA complex was detected by immunofluorescence staining. The white arrows indicate colocalization of EB1 with P[Y418] Src or APC. Scale bars: 50 μm. Quantification analysis of colocalization of EB1 with p[Y418] Src (D) and APC (E) was shown. Mean±s.d., **P<0.01.