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. 2014 Sep 11;16(3):328–337. doi: 10.1016/j.chom.2014.08.009

Figure 2.

Figure 2

Characterization of Binding between HKU4-RBD and hCD26 by Flow Cytometry

(A) Huh7 cells stained with an anti-hCD26 antibody.

(B) Huh7 cells stained with MERS-RBD, HKU4-EBD, or HKU5-RBD.

(C) Huh7 cells stained with HKU4-RBD in the presence of an anti-hCD26 antibody or an isocontrol antibody.

(D) Huh7 cells stained with HKU4-RBD in the presence of hCD26 (hCD26-ecto) or hACE2 (hACE2-ecto) ectodomain protein.

(E) BHK cells stained with an anti-hCD26 antibody.

(F) BHK cells stained with MERS-RBD, HKU4-RBD, or HKU5-RBD.

(G) hCD26-transfected BHK cells stained with an anti-hCD26 antibody.

(H) hCD26-transfected BHK cells stained with MERS-RBD, HKU4-RBD, or HKU5-RBD.