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. 2014 Sep 11;16(3):328–337. doi: 10.1016/j.chom.2014.08.009

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Copyright © 2014 Elsevier Inc. All rights reserved.

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Huh7 Infection by Lentiviral Particles Pseudotyped with BatCoV HKU4 S

(A) Proteolytic processing of the embedded BatCoV HKU4 S by trypsin. The HKU4 pseudovirus was treated with trypsin at the indicated concentrations and characterized with an antibody recognizing a FLAG epitope engineered at the S C terminus. The untreated MERS pseudovirus was included as a reference control.

(B) A Huh7 cell infection assay with the indicated pseudoviruses.

(C) An antibody blocking assay using HKU4 pseudoviruses treated with 10 μg/ml trypsin and an anti-hCD26 antibody. The recorded fluorescence intensities were plotted as histograms, and the error bars represent ± SD for triplicate experiments.