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Optimization of probe immobilization concentration. ATP5Oc was immobilized on the aerogel surface in 10, 1, and 0.1 μM. The target DNA, cy3-ATP5O, was titrated in 160, 80, 40, and 20 nM. For each probe concentration, slides were scanned before and after the probe-target hybridization was performed. Positive and negative controls, which guarantee the validation of result, were set by 10 μM Sp5-cy3 and hybridization buffer, respectively.
