Skip to main content
. Author manuscript; available in PMC: 2020 Jul 20.
Published in final edited form as: Nat Med. 2020 Jan 20;26(2):236–243. doi: 10.1038/s41591-019-0733-7

Figure 2. Circulating DiHS/DRESS T cells with distinct transcriptomic profiles and enrichment of human herpesvirus 6b DNA in CD4+ central memory T cells.

Figure 2.

a, scRNAseq analysis on PBMCs from DiHS/DRESS (6,956 cells) and an age- and sex-matched HV (7,976 cells). Individual cells are color-coded based on cluster (n=19) in a tSNE plot. tSNE projections of well-known marker genes (bottom). b, Numbers of differentially expressed genes (nDEGs) between DiHS/DRESS and HV clusters. DEG: |log fold change| > 0.5, adjusted p-value < 0.05, Wilcoxon rank sum test. c, tSNE projections of selected genes, segregated by origin. Dotted region highlights clusters with high nDEGs (CD4(3), CD8(1), and mitotic cluster). d, e, Flow cytometry analysis of PBMCs for chemokine receptor expression and memory phenotype (a representative of two independent experiments). f, Violin plots show the distributions of JAK3, STAT1, and IL2RG expression levels in clusters with high transcriptomic changes (CD4(3), CD8(1), and mitotic cluster, DiHS/DRESS, n=925 cells; HV, n=2,960 cells). Numbers indicate percentages of cells that express each gene. g, Quantitative RT-PCR of human herpesviruses (HHV) in PBMC. h, Quantitative PCR for HHV6b DNA using sorted PBMC subsets. g,h, n=1. a representative of two independent sampling point.