Skip to main content
. Author manuscript; available in PMC: 2020 Nov 18.
Published in final edited form as: J Vis Exp. 2020 Jan 18;(155):10.3791/60571. doi: 10.3791/60571

Figure 2: In vitro differentiation of adapted single-cell type hESCs into three germ layers.

Figure 2:

(A) Representative phase images of embryonic bodies (EB) derived from single-cell type of hESCs. Scale bar = 100 μm. (B) Immunofluorescent images of differentiated hESCs analyzed for the expression of the three different germ layer markers: SOX17 (endoderm), SMA (mesoderm), and Tuj-1 (ectoderm). Nuclei were stained with DAPI. Scale bar = 50 μm.