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. Author manuscript; available in PMC: 2020 Nov 18.
Published in final edited form as: J Vis Exp. 2020 Jan 18;(155):10.3791/60571. doi: 10.3791/60571

Figure 4: hESC culture on different basement membrane matrix products.

Figure 4:

(A) hESCs cultured on Matrigel or Geltrex exhibited an ability to grow and differentiate into NPCs that was similar to the single cell-culture. Cells were stained with a NESTIN antibody. Nuclei were stained with DAPI. Scale bar = 50 μm. (B) hESCs cultured on Matrigel or Geltrex showed similar potential to differentiate into NPCs as indicated by the percentage of NESTIN- and PAX6-positive cells. Cells were analyzed by flow cytometry at day 7 of NPC differentiation.