Table 1.
Antibodies used in immunocytochemistry and FACS analysis.
| Primary Antibodies | Species | Dilution | Catalog Number | Company |
|---|---|---|---|---|
| OCT4 | Mouse | 1:1000 | sc-5279 | Santa Cruz |
| TRA 1-81 | Mouse | 1:500 | sc-21706 | Santa Cruz |
| SSEA-1 | Mouse | 1:500 | sc-21702 | Santa Cruz |
| SOX1 | Goat | 1:500 | AF-3369 | R&D |
| SOX2 | Rabbit | 1:1000 | sc-20088 | Santa Cruz |
| SMA | Rabbit | 1:500 | ab-5694 | Abcam |
| Tuj1 | Mouse | 1:1000 | T8578 | Sigma |
| NESTIN | Mouse | 1:1000 | ab-22035 | Abcam |
| OTX2 | Mouse | 1:250 | ab-21990 | Abcam |
| hNCAM | Mouse | 1:200 | sc-106 | Santa Cruz |
| hPAX6 | Mouse | 1:250 | 561664 | BD |
| TH | Mouse | 1:500 | T1299 | Sigma |
| S100b | Mouse | 1:250 | ab4066 | Abcam |
| GFAP | Rabbit | 1:1000 | ab7260 | Abcam |
| Secondary Antibodies | ||||
| Anti-mouse | Goat | 1:1000 | AF 488 or 647 | Life Technologies |
| Anti-rabbit | Goat | 1:1000 | AF 488 or 647 | Life Technologies |