Table 1.
Identification results of the targeted proteins.
| Protein name and lengtha | Theoretical MW (Da) | Observed MW (Da) | Sequence coverage (%) | Mascot score | Identified peptides | Protein sequence |
|---|---|---|---|---|---|---|
| SAA1α (1–104) | 11,682.7 | 11,682.9 | 80.77 | 102 | -.RSFFSFLGEAFDGAR.D. R.SFFSFLGEAFDGAR.D. R.EANYIGSDKYFHAR.G. K.RGPGGVWAAEAISDAR.E. R.GPGGVWAAEAISDAR.E. R.FFGHGAEDSLADQAANEWGR.S. K.DPNHFRPAGLPEKY.- | RSFFSFLGEAFDGARDMWRAYSDMREANYIGSDKYFHARGNYDAAKRGPGGVWAAEAISDARENIQRFFGHGAEDSLADQAANEWGRSGKDPNHFRPAGLPEKY. |
| SAA1α (2–104) | 11,526.5 | 11,526.6 | 81.55 | 104 | R.SFFSFLGEAFDGAR.D. R.DMWRAYSDMR.E. R.AYSDMR.E. R.EANYIGSDKYFHAR.G. K.RGPGGVWAAEAISDAR.E. R.GPGGVWAAEAISDAR.E. R.FFGHGAEDSLADQAANEWGR.S. K.DPNHFRPAGLPEKY.- | SFFSFLGEAFDGARDMWRAYSDMREANYIGSDKYFHARGNYDAAKRGPGGVWAAEAISDARENIQRFFGHGAEDSLADQAANEWGRSGKDPNHFRPAGLPEKY. |
| SAA1α (3–104) | 11,439.4 | 11,439.5 | 67.65 | 72 | R.EANYIGSDKYFHAR.G. K.RGPGGVWAAEAISDAR.E. R.GPGGVWAAEAISDAR.E. R.FFGHGAEDSLADQAADEWGR.S. K.DPNHFRPAGLPEKY.- | FFSFLGEAFDGARDMWRAYSDMREANYIGSDKYFHARGNYDAAKRGPGGVWAAEAISDARENIQRFFGHGAEDSLADQAANEWGRSGKDPNHFRPAGLPEKY |
| SAA1β (2–104) | 11,584.6 | 11,583.7 | 100 | 145 | -.SFFSFLGEAFDGAR.D. -.SFFSFLGEAFDGARDMWR.A. R.AYSDMR.E. R.EANYIGSDKYFHAR.G. K.YFHAR.G. K.YFHARGNYDAAK.R. K.RGPGGAWAAEVISDAR.E. R.GPGGAWAAEVISDAR.E. R.GPGGAWAAEVISDARENIQR.F. R.FFGHDAEDSLADQAANEWGR.S. R.SGKDPNHFRPAGLPEK.Y. K.DPNHFRPAGLPEKY.- | SFFSFLGEAFDGARDMWRAYSDMREANYIGSDKYFHARGNYDAAKRGPGGAWAAEVISDARENIQRFFGHDAEDSLADQAANEWGRSGKDPNHFRPAGLPEKY. |
| SAA1γ (1–104). | 11,654.7 | 11,655.1 | 84.62 | 90 | -.RSFFSFLGEAFDGAR.D. R.SFFSFLGEAFDGAR.D. R.AYSDMR.E. R.EANYIGSDKYFHAR.G. K.RGPGGAWAAEAISDAR.E*. R.FFGHGAEDSLADQAANEWGR.S. R.SGKDPNHFRPAGLPEK.Y. K.DPNHFRPAGLPEKY.- | RSFFSFLGEAFDGARDMWRAYSDMREANYIGSDKYFHARGNYDAAKRGPGGAWAAEAISDARENIQRFFGHGAEDSLADQAANEWGRSGKDPNHFRPAGLPEKY. |
| SAA2α (1–104) | 11,628.7 | 11,627.3 | 76.92 | 84 | R.SFFSFLGEAFDGAR.D. R.AYSDMR.E. R.AYSDMREANYIGSDK.Y. K.RGPGGAWAAEVISNAR.E. R.GPGGAWAAEVISNAR.E. R.LTGHGAEDSLADQAANK.W. R.SGRDPNHFRPAGLPEK.Y. R.DPNHFRPAGLPEK.Y. R.DPNHFRPAGLPEKY.- | RSFFSFLGEAFDGARDMWRAYSDMREANYIGSDKYFHARGNYDAAKRGPGGAWAAEVISNARENIQRLTGHGAEDSLADQAANKWGRSGRDPNHFRPAGLPEKY |
| SAA2α (3–104) | 11,385.4 | 11,387.1 | 49.02 | 64 | R.EANYIGSDKYFHAR.G. K.RGPGGAWAAEVISNAR.E. R.GPGGAWAAEVISNAR.E. R.LTGHGAEDSLADQAANKWGR.S | FFSFLGEAFDGARDMWRAYSDMREANYIGSDKYFHARGNYDAAKRGPGGAWAAEVISNARENIQRLTGHGAEDSLADQAANKWGRSGRDPNHFRPAGLPEKY |
| SAA2α (2–104) | 11,472.5 | 11,472.9 | 84.47 | 104 | R.SFFSFLGEAFDGAR.D. R.AYSDMR.E. R.EANYIGSDKYFHAR.G. K.RGPGGAWAAEVISNAR.E. R.GPGGAWAAEVISNAR.E. R.LTGHGAEDSLADQAANK.W. R.LTGHGAEDSLADQAANKWGR.S. R.SGRDPNHFRPAGLPEK.Y. R.DPNHFRPAGLPEKY.- | SFFSFLGEAFDGARDMWRAYSDMREANYIGSDKYFHARGNYDAAKRGPGGAWAAEVISNARENIQRLTGHGAEDSLADQAANKWGRSGRDPNHFRPAGLPE KY |
| SAA2β (1–104) | 11,647.8 | 11,647.3 | 78.85 | 100 | -.RSFFSFLGEAFDGAR.D. R.SFFSFLGEAFDGAR.D. R.EANYIGSDKYFHAR.G. K.RGPGGAWAAEVISNAR.E. R.GPGGAWAAEVISNAR.E. R.LTGHGAEDSLADQAANKWGR.S. R.SGRDPNHFRPAGLPEK.Y. R.DPNHFRPAGLPEKY.- | RSFFSFLGEAFDGARDMWRAYSDMREANYIGSDKYFHARGNYDAAKRGPGGAWAAEVISNARENIQRLTGRGAEDSLADQAANKWGRSGRDPNHFRPAGLPEKY |
| SAA2β (2–104) | 11,491.6 | 11,491.3 | 62.14 | 94 | R.SFFSFLGEAFDGAR.D. R.AYSDMR.E. R.EANYIGSDKYFHAR.G. K.RGPGGAWAAEVISNAR.E. R.GPGGAWAAEVISNAR.E. R.DPNHFRPAGLPEK.Y. R.DPNHFRPAGLPEKY.- | SFFSFLGEAFDGARDMWRAYSDMREANYIGSDKYFHARGNYDAAKRGPGGAWAAEVISNARENIQRLTGRGAEDSLADQAANKWGRSGRDPNHFRPAGLPEKY |
aProtein lengths were calculated for mature proteins without signal sequences. Arginine (R) was considered as the first amino acid of the N-terminal of the protein.