Table 2.
Comparison for depletion methods of high-abundant proteins in plasma
| Classification | Methods | Advantage | Disadvantage |
|---|---|---|---|
| Affinity based | Cibracon Blue and related dye | -Low cost | -Lack of specificity |
| -High loading capacity | |||
| Protein A/G | -High affinity for immunoglobulins | -Possible to remove only immunoglobulins | |
| -Not bind all of the immunoglobulin subgroups | |||
| Peptide-based ligands | -Low cost | ||
| -High reproducibility | |||
| -Sterilizable | |||
| -Reactivity across species | |||
| Size based | Centrifugal ultrafiltration | -Convenient | -Low effectiveness |
| -Low cost | -Low reproducibility | ||
| Antibody based | Monoclonal antibody | -High specificity | -Fail to remove the related fragments of target proteins |
| Polyclonal antibody | |||
| IgG-based system | -Depletion of multiple proteins in one step | -Lack of reactivity across multiple mammalian species | |
| -High affinity and high specificity | -High cost | ||
| -Low sample capacity | |||
| IgY-based system | -Depletion of multiple proteins in one step | -High cost | |
| -High affinity and high specificity | -Low sample capacity | ||
| -High reactivity across multiple mammalian species | |||