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Method of separation of major low molecular compounds (LMC) from S-fraction
| (1) | 100 g of S-fraction dried powder was dissolved in 5000 ml water, and this was applied to a column on which 5000 g Diaion HP20 had been packed |
| (2) | The adsorbed S-fraction with Diaion HP20 was successively eluted with 5000 ml of water, 5000 ml of 50% aqueous methanol and 5000 ml of 100% methanol |
| (3) | Each elution was evaporated to dryness |
