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. Author manuscript; available in PMC: 2020 Mar 30.
Published in final edited form as: J Proteome Res. 2019 Jul 23;18(9):3404–3418. doi: 10.1021/acs.jproteome.9b00324

Figure 4.

Figure 4.

Localization of the PHIST protein in late liver stage parasites and merosomes. (A) Immunofluorescence staining of late liver stage parasites at 60 h postinfection indicates the PHIST protein (PBANKA_1145400) partially colocalizes with the parasitophorous vacuole marker UIS4. Colocalized pixels in white. DNA stained with Hoechst. (B,C) Immunofluorescence staining of merosomes and free merozoites at 65 h postinfection demonstrates the PHIST protein associates with individual liver stage merozoites. No UIS4 staining was detected in the mature merosome or free hepatic merozoite shown.