Table 4.
Proteins with Processed PEXEL Protein Export Motifs
| PlasmoDB ID | protein | peptidea | SPb | repsc | PSMsd |
|---|---|---|---|---|---|
| PBANKA_0208900 | StAR-related lipid transfer protein, putative | RIL.EEALMDVENVKENLK | Y | 3 | 95 |
| PBANKA_1400700 | Plasmodium exported protein | RHL.AEYIPNISNPTGNVYHFSR | N | 3 | 56 |
| PBANKA_1003000 | liver specific protein 2 (LISP2) | RIL.AETQHENLVLSTDKDKER | Y | 3 | 23 |
| PBANKA_1003000 | liver specific protein 2 (LISP2) | RLI.AEKESENNSENNSQDVK | Y | 2 | 14 |
| PBANKA_1328000 | serine/threonine protein phosphatase (UIS2) | RVL.QEQNEDIKDDNDENDEGDEEDEYYSYLK | Y | 3 | 7 |
| PBANKA_1365500 | exported protein IBIS1 | RIL.SELDQNKDQNLGYK | YR | 3 | 7 |
| PBANKA_1465000 | fam-c protein | RSL.SEHSTEDKEETVTISDNK | Y | 2 | 4 |
| PBANKA_0700700 | Plasmodium exported protein, unknown function | RIL.SSMSNEKPYNILR* | Y | 2 | 4 |
| PBANKA_0100600 | schizont membrane associated cytoadherence protein (SMAC) | RFL.VEYYDANIDQYNGNQSK | Y | 2 | 3 |
| PBANKA_1145400 | Plasmodium exported protein (PHIST) | RNL.SETSVVNDNLSNNVNNLRDEPK | Y | 1 | 6 |
a
Identified peptide sequence bearing a cleaved and acetylated PEXEL motif [K/R]X[L/I]X[D/E/Q] or possible PEXEL motif [K/R]X[L/I]XX (denoted by “*”). For both canonical and possible PEXEL motifs, the point of cleavage is denoted with “.”, and the three residues in the protein sequence N-terminal to the peptide are shown. All displayed peptides were observed with mass spectral evidence for N-terminal acetylation.
b
Signal peptide prediction; YR indicates a recessed signal peptide.73
c
Number of biological replicates in which the cleaved and acetylated PEXEL peptide was identified.
d
Total number of PSMs that identified the cleaved and acetylated PEXEL across all three biological replicates.