Figure 5.

Chronic effect of leptin on lipids and MTP expression in the intestine and liver of WT mice fed a Western diet for 2 months. C57BL/6J mice on a Western diet (n = 4) were injected daily with 0.05 mg/kg body weight of leptin for 2 months. Mice were fasted for 4 h after the last injection. Plasma was used to measure the levels of triglycerides (A), total cholesterol (B), HDL cholesterol (C), and non-HDL cholesterol (D). Triglycerides (E and G) and cholesterol (F and H) were also measured in the intestines (E and F) and livers (G and H) of these mice. Intestinal and hepatic tissues were also used to measure the activity (I and K) and the mRNA (J and L) levels of MTP. Data are plotted as mean ± S.D. *, p < 0.05; **, p < 0.01; and ***, p < 0.001 were determined by Student's t test.