Figure 5.

Secretion of vesicular bodies in CAP59Δ and CAP67Δ mutants. A, incubation of CAP67Δ with BODIPY FL C₁₂ (lipophilic dye, red) and the R.E probe shows vesicle secretion (arrows). B, CAP59Δ cells incubated with BODIPY TR (lipophilic dye, red) also show signs of secretion of glycan-containing vesicles (inset and arrows). C, CAP67Δ cells incubated with the BODIPY TR and R.E probe show signs of colocalization; however, BODIPY TR also stains nonglycan-containing vesicles (arrows) and shows signs of secretion of nonglycan-containing vesicular bodies (arrow). Scale denoted in bright field: A, 5 μm; B, 7 μm (inset, 5 μm); and C, 5 μm.