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. 2020 Feb 20;295(13):4289–4302. doi: 10.1074/jbc.RA119.010491

Figure 6.

Figure 6.

Effect of cholesterol depletion on the localization of angulin-1. A, immunofluorescence staining of EpH4 cells (control) and cholesterol-depleted cells after MβCD treatment using antibodies against tTJ-associated proteins (angulin-1, tricellulin) and TJ-associated proteins (occludin, claudin-3, and ZO-1). Scale bar, 20 μm. B, quantitation of the TC enrichment of angulin-1 or tricellulin upon MβCD treatment. The graph represents mean ± S.D. (error bars) (n = 3–4 each). **, p < 0.005, compared by t test; n.s., not significant; a.u., arbitary units. C, immunoblotting of lysates from EpH4 cells (control) and cholesterol-depleted cells after MβCD treatment using antibodies against tTJ-associated proteins (angulin-1 and tricellulin) and TJ-associated proteins (occludin, claudin-3, and ZO-1). IB, immunoblotting.