Figure 4.

Disruption of FOXO1 diminished DOX-induced cardiomyocyte apoptosis. A and B, NRCMs were transfected with siControl or siFOXO1 prior to treatment with DOX (1 μm) for 24 h. A, protein levels were measured by Western blotting. B, apoptosis was evaluated by staining with TUNEL (green), cTnT (red), and nuclei (DAPI, blue). Scale bar = 20 μm. Two-tailed Student's t test. **, p < 0.01 versus siControl. C and D, NRCMs were treated with DOX (1 μm) in the presence of the FOXO1 inhibitor AS1842856 (1 μm) or vehicle for 24 h. C, protein levels were measured by Western blotting. D, apoptosis was evaluated by TUNEL staining. Two-tailed Student's t test. **, p < 0.01 versus vehicle.