Table 3.

Kinetic constants for in vitro reactivation by LG bis-oximes and RS194B monoxime

OP-hAChE conjugates were obtained by inhibition with paraoxon and analogues of sarin, cyclosarin, and VX. Maximal reactivation rate constant k₂(min⁻¹), Michaelis–Menten type constant K_ox (mm), and the overall second-order reactivation rate constant k_r(m⁻¹ min⁻¹) were calculated by non-linear regression from curves in Fig. 6 as described previously (7). S.E. values of constants k₂ and k_r obtained from regression were typically smaller than 30% of the reported values. Constants for 2PAM (in the absence of DMSO (7)) are listed for comparison (gray shading). Different shading for LG bis-oximes indicates difference in the central heterocycle (yellow, homopiperazines; white, piperazines; blue, piperidines). Due to 1% DMSO in the reactivation assay, values of constants for RS194B do not correspond exactly to our previously published ones (7), which were obtained at lower DMSO concentration.