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. 2019 Nov 5;295(13):4065–4078. doi: 10.1074/jbc.RA119.009827

Figure 2.

Figure 2.

SLC2A3 and NICI are co-regulated by HIF-1. a, relative NICI and SLC2A3 RNA expression levels (compared with untreated control) in a selection of human cell lines exposed to 1 mm DMOG for 16 h as measured by qPCR (mean ± S.D. from three independent experiments per cell line). Statistical analyses were performed using the one-sample t test (*, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001). b, ChIP-Seq analysis in PTCs confirms the single HIF-binding site at the CAT1466.1/NICI locus ∼35 kb upstream of SLC2A3. Expression of NICI and SLC2A3 is induced upon HIF-stabilization with 1 mm DMOG in RNA-Seq experiments. c, mRNA expression of NICI and SLC2A3 in a collection of 16 PTC cultures left untreated or treated with 1 mm DMOG for 16 h. d, expression levels of NICI correlate well with those of SLC2A3 in DMOG-treated PTC. e, time course of relative expression levels of NICI and SLC2A3 RNA in PTC exposed to 1 mm DMOG for the indicated time. Data are normalized to time point 0 h and bars represent mean ± S.D. measured in cells from n = 3 individuals.