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. 2019 Nov 5;295(13):4065–4078. doi: 10.1074/jbc.RA119.009827

Figure 6.

Figure 6.

NICI mediates hypoxic SLC2A3 induction. a, the site within the NICI transcript targeted by the guide RNA for CRISPR/Cas9-mediated mutation is highlighted in green (NICI mut). b, HIF ChIP-qPCR for the NICI HRE and the control HRE in the EGLN3 intronic region. DNA fragments were captured by ChIP using HIF-1β antibodies or serum control in clones with mutations in the NICI transcript (NICI mut) n = 4 independent clones) or control clones (control; n = 4 independent clones). Cells were exposed to 1 mm DMOG for 16 h. c, expression qPCR for NICI and SLC2A3 in untreated or DMOG-treated clones with (NICI mut; n = 9 independent clones) or without mutations in the NICI transcript (control; n = 5 independent clones). Results are from three independent experiments for each clone of cells. d, RNA polymerase 2 (RNApol2) DNA interactions were determined by ChIP qPCR at the NICI HRE and the SLC2A3 transcriptional start site (TSS) in NICI transcript-mutated clones (n = 4) compared with control clones (n = 4). Cells were exposed to 1 mm DMOG for 16 h.