Figure 7.

NICI regulates glucose consumption and proliferation. a and b, glucose consumption (a) and lactate (b) in culture media from hypoxic cells. Cells (n = 2 for HRE mut, n = 3 for NICI mut, n = 4 for mut control) were cultured in 1 mm DMOG for 24 h before glucose consumption and lactate were measured in the medium. c, MTS proliferation assay with cells from a performed in triplicates per time point under hypoxic conditions (1% O₂). Statistical analyses were performed using the one- or two-sample t test (*, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001) comparing values from NICI/HRE mutated cells to the respective values from control clones (mut control).