Figure 6.

MV-enriched fraction from SP induces a potent transcriptional response and decidualization in eSFs. (A) eSFs were treated with media alone, E₂P₄, the MV-enriched fraction or E₂P₄ + the MV-enriched fraction. After 4 or 12 days of treatment, culture supernatants were assessed for decidualization by measuring IGFBP1 levels by ELISA. Each value corresponds to the mean ± SD of cultures set up in triplicates. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 and ^****P < 0.0001 as determined using a two-tailed Student’s t-test comparing each treatment condition (E₂P₄, the MV-enriched fraction or E₂P₄ + the MV-enriched fraction) to the mock-treated samples. (B) PCA of RNAseq data from eSFs treated for 6 h with vehicle, the MV-enriched fraction or reconstituted SP. (C) Heatmap illustrating hierarchical clustering of eSFs treated with vehicle, the MV-enriched fraction, or reconstituted SP, based on the RNAseq datasets. Hierarchical clustering was based on differentially expressed genes across treatment (281 genes), not taking donor ID into account. (D) Venn diagram showing the number of transcripts induced by the MV-enriched fraction and reconstituted SP.