Figure 7.

CRISPR/Cas-9 mediated knockout of IL-11 receptor reveals IL-11 signaling is important for SP-enhanced decidualization. (A) Average percentage of indels and knockout (KO) efficiency at the interleukin-11 receptor (IL-11RA) locus in eSFs treated with the IL-11RA guide or the scrambled control, as determined by ICE analyses following Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas-9-mediated gene editing. (B) IL-11RA KO eSFs, scrambled negative control eSFs and unedited (untreated) eSFs were treated with media alone, E₂P₄, 10% SP or E₂P₄ + 10% SP. After 4 or 12 days of treatment, culture supernatants were assessed for decidualization by measuring IGFBP1 levels by ELISA. Each value corresponds to the mean ± SD of cultures set up in triplicates. ^*P < 0.05, ^**P < 0.01 and ^****P < 0.0001, as determined by two-tailed Student’s t-test, indicate a significant decrease in IGFBP1 levels in the IL-11RA KO as compared with the scrambled negative control.