Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Rabbit polyclonal anti-NMIIA | Sigma-Aldrich | Cat# M8064, RRID:AB_260673 | WB_1:1000; IF_1:200 |
| Antibody | Rabbit polyclonal anti-NMIIB | Sigma-Aldrich | Cat# M7939, RRID:AB_260669 | WB_1:1000; IF_1:200 |
| Antibody | Rabbit polyclonal canti-α-adducin | Abcam | Cat# ab51130, RRID:AB_867519 |
WB_1:1000 |
| Antibody | Rabbit monoclonal anti-vinculin | Abcam | Cat# ab129002, RRID:AB_11144129 | WB_1:1000 |
| Antibody | Mouse monoclonal anti-βactin | Sigma-Aldrich | Cat# A5441, RRID:AB_476744 | WB_1:5000 |
| Antibody | Mouse monoclonal anti-αtubulin | Sigma-Aldrich | Cat# T6199, RRID:AB_477583 | WB_1:1000 |
| Antibody | Peroxidase-AffiniPure donkey polyclonal anti-rabbit IgG (H+L) |
Jackson Immuno Research Labs | Cat# 711-035-152, RRID:AB_10015282 | WB_1:5000 |
| Antibody | Peroxidase-AffiniPure donkey polyclonal anti-mouse IgG (H+L) | Jackson Immuno Research Labs | Cat# 715-035-151, RRID:AB_2340771 | WB_1:5000 |
| Antibody | Mouse monoclonal anti-βII-spectrin | BD Transduction | Cat# 612563,RRID:AB_399854 | IF_1:200 |
| Antibody | Rabbit polyclonal anti-MAP2 | Synaptic Systems | Cat# 188002, RRID:AB_2138183 | IF_1:20000 |
| Antibody | Rabbit polyclonal anti-NMIIC | Robert Adelstein NHLBI, Bethesda, USA | N/A | IF_1:40 |
| Antibody | Rabbit polyclonal anti-pMLC2 Thr18/Ser19 | Cell Signaling | Cat# 3674, RRID:AB_2147464 | IF_1:50 |
| Antibody | Rabbit polyclonal anti-MYPT1 | Cell Signaling | Cat# 2634, RRID:AB_915965 | IF_1:50 |
| Antibody | Rabbit monoclonal anti-MLC2 | Cell Signaling | Cat# 1678505S | IF_1:40 |
| Antibody | Goat polyclonal anti-mouse STAR 635P | Abberior GmbH | Cat# 2-0002-007-5 | IF_1:200 |
| Antibody | Goat polyclonal anti-rabbit STAR 635P | Abberior GmbH | Cat# 2-0012-007-2 | IF_1:200 |
| Antibody | Goat polyclonal anti-mouse STAR 580 | Abberior GmbH | Cat# 2-0002-005-1, RRID:AB_2620153 | IF_1:200 |
| Antibody | Goat polyclonal anti-mouse STAR 580 | Abberior GmbH | Cat# 2-0012-005-8 | IF_1:200 |
| Antibody | Goat polyclonal anti- mouse Alexa Fluor 532 | Thermo Fischer Scientific | Cat# A-11002, RRID:AB_2534070 | IF_1:200 |
| Antibody | Donkey polyclonal anti- rabbit Alexa Fluor 647 | Jackson ImmunoResearch | Cat# 711-605-152 | IF_1:1000 STORM_ 1:200 |
| Chemical compound, drug | Blebbistatin | Sigma-Aldrich | Cat# B0560 | 3 µM |
| Chemical compound, drug | ML-7 | Sigma-Aldrich | Cat# I2764 | 4 µM |
| Chemical compound, drug | Myovin1 | Calbiochem | Cat# 475984 | 4 µM |
| Chemical compound, drug | Calyculin A | Sigma-Aldrich | Cat# C5552 | 5 nM |
| Chemical compound, drug | Dimethyl sulfoxide (DMSO) | VWR International | Cat# A3672.0050 | N/A |
| Chemical compound, drug | Phalloidin 635P | Abberior GmbH | Cat# 2-0205-002-5 | 0.33 µM |
| Cell line | CAD (mouse) | ECACC through Sigma-Aldrich |
Cat# 08100805, RRID:CVCL_0199 | N/A |
| Cell line | PC-12 (rat) | ATCC | Cat# CTL-1721, RRID:CVCL_F659 | N/A |
| Cell line | SH-SY5Y (human) | ATCC | Cat# CRL-2266, RRID:CVCL_0019 | N/A |
| Recombinant DNA reagent | pEGFP-C1 | Addgene | N/A | N/A |
| Recombinant DNA reagent | pLKO.1 | Addgene | N/A | N/A |
| Recombinant DNA reagent | shRNA NMIIA | Rai et al., 2017 | N/A | 5’GCGATACTACTCAGGGCTTAT3’ |
| Recombinant DNA reagent | shRNA NMIIB | This paper | N/A | 5‘GCCAACATTGAAACATACCT3’ |
| Recombinant DNA reagent | shRNA NMIIC | This paper | N/A | 5’CCGGGCTCATTTATACCTACT3’ |
| Recombinant DNA reagent | shRNA RLC | Wang et al., 2008 | N/A | 5′GCACGGAGCGAAAGACAAA3′ |
| Recombinant DNA reagent | shRNA MYPT1 | This paper | N/A | 5′GAGCCTTGATCAGAGTTATAAC3′ |
| Recombinant DNA reagent | shRNA α-adducin | Sigma-Aldrich | Cat# TRCN0000108809 | 5’GCAGAAGAAGAGGGTGTCTAT3’ |
| Recombinant DNA reagent | Human mutated ShRNA-resistant NMIIA | This paper | N/A | Vectorbuilder Page 20_line 374–376 |
| Recombinant DNA reagent | Human mutated ShRNA-resistant NMIIB | This paper | N/A | Vectorbuilder Page 20_line 374–376 |
| Recombinant DNA reagent | Human ShRNA-resistant RLC | Addgene | Cat #35680 | N/A |
| Recombinant DNA reagent | Human mutated ShRNA-resistant MYPT1 | This paper | N/A | Vectorbuilder Page 20_line 374–376 |
| Recombinant DNA reagent | CMV-eGFP-NMIIA | Addgene | Cat #11347 | N/A |
| Recombinant DNA reagent | CMV-NMIIA-mApple | John Hammer, NHLBI, Bethesda, USA | N/A | N/A |
| Commercial assay or kit | NZY Total RNA Isolation Kit | NZY Tech | Cat# MB13402 | N/A |
| Commercial assay or kit | SuperScript First-Strand Synthesis System for RT-PCR | Thermo Fisher Scientific | Cat# 11904018 | N/A |
| Sequence-based reagent | NMIIC sense primer | This paper | N/A | 5’CCTGGCTGAGTTCTCCTCAC3’ |
| Sequence-based reagent | NMIIC antisense primer | This paper | N/A | 5’TGCTTCTGCTCCATCATCTG3’ |
| Sequence-based reagent | RLC sense primer | This paper | N/A | 5’CCTTTGCCTGCTTTGATGAG3’ |
| Sequence-based reagent | RLC antisense primer | This paper | N/A | 5’GTGACTGGGATGGGGTGTAG3’ |
| Sequence-based reagent | MYPT1 sense primer | This paper | N/A | 5’AAGGGAACGAAGAGCTCTAGAAA3' |
| Sequence-based reagent | MYPT1 antisense primer | This paper | N/A | 5’TGACAGTCTCCAGGGGTTCT3’ |
| Sequence-based reagent | β-actin sense primer | This paper | N/A | 5’ACCACACCTTCTACAATGAG3’ |
| Sequence-based reagent |
β-actin antisense primer | This paper | N/A | 5’TAGCACAGCCTGGATAGC3’ |
| Sequence-based reagent | GADPH sense primer | This paper | N/A | 5’AGGCACCAAGATACTTACAAAAAC3’ |
| Sequence-based reagent | GADPH antisense primer | This paper | N/A | 5’TGTATTGTAACCAGTCATCAGCA3' |
| Software, algorithm | MATLAB R2018a | MATLAB | RRID:SCR_001622 | N/A |
| Software, algorithm |
Fiji | NIH | SRRID:SCR_002285 | N/A |
| Software, algorithm | Leica LAS X software | Leica | RRID:SCR_013673 | N/A |
| Software, algorithm | µSpikeHunter software | Heiney et al., 2019 | N/A | N/A |
| Software, algorithm | GraphPad Prism | GraphPad | RRID:SCR_002798 | N/A |
| Software, algorithm | Huygens Software | Scientific Volume Imaging | RRID:SCR_014237 | N/A |