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. 2020 Mar 30;9:e53387. doi: 10.7554/eLife.53387

Figure 10. LIN-28 coordinates both lin-29a and lin-29b regulation.

(A) Confocal images of endogenously tagged LIN-29 protein isoforms in wild-type or lin-28(n719) background in the epidermis (strains HW1822, HW1826, HW1835, HW1924, HW1925, HW1926). Animals were grown at 25°C for 20 hr (control strains) and 22 hr (lin-28(n719) strains) to reach an equivalent early L3 developmental stage. Arrows indicate seam cell, arrowheads hyp7 nuclei. Scale bars: 10 μm. (B) RT-qPCR analysis to measure the -∆∆Ct of lin-29a mRNA levels in wild-type and lin-28(n719) mutant background (normalized by act-1 mRNA levels) over time. (C) RT-qPCR analysis to measure the -∆∆Ct of lin-29b mRNA levels in wild-type and lin-28(n719) mutant background (normalized by act-1 mRNA levels) over time.

Figure 10.

Figure 10—figure supplement 1. Expression of lin-29a and lin-29b in wild-type and lin-28 mutant animals over time.

Figure 10—figure supplement 1.

(A–C) Confocal images of endogenously tagged LIN-29 protein isoforms in wild-type (lin-28(+)) or lin-28(n719) background in the epidermis (strains HW1822, HW1826, HW1835, HW1924, HW1925, HW1926). Animals were grown at 25°C for 20 hr, 22 hr and 24 hr (A, B, and C, resp., for control strains) and 22 hr, 24 hr and 26 hr (A, B, and C, resp., for lin-28(n719) strains) to reach an equivalent developmental stage. Arrows indicate seam cell, arrowheads hyp7 nuclei. Scale bars: 10 μm.