Skip to main content
. 2020 Mar 30;9:e53387. doi: 10.7554/eLife.53387

Figure 7. Spatial and temporal expression pattern of LIN-29a and LIN-29b.

(A) Western blot of C-terminally GFP::3xFLAG-tagged endogenous LIN-29a and LIN-29b proteins in different developmental stages using an anti-FLAG antibody. Actin-1 is used as a loading control. The asterisk indicates a band of unclear origin. (B–D) Confocal images of endogenously tagged LIN-29 isoforms in the epidermis of animals at the indicated developmental stages, which were confirmed by examination of gonad development. Arrows indicate seam cell, arrowheads hyp7 nuclei. Scale bars: 10 μm.

Figure 7.

Figure 7—figure supplement 1. Expression of lin-29 isoforms.

Figure 7—figure supplement 1.

(A) Confocal images of endogenously tagged LIN-29 protein isoforms in the region of the vulva and the uterus at the indicated developmental stages. At the L2-to-L3 molt (A), lin-29a is expressed in the anchor cell (AC), while lin-29b is weakly expressed in the sex myoblasts (SMs). In mid-L3 stage worms, the six daughters of the VPCs P5.p-P7.p express lin-29b. At the late L3 stage, lin-29b is strongly expressed in the sex myoblast (SM) daughters and all 12 granddaughters of the VPCs P5.p-P7.p, while LIN-29a specifically accumulates in the granddaughters of P5.p and P7.p, but not in those of P6.p. Scale bars: 10 μm. (B) Confocal images of endogenously tagged LIN-29 protein isoforms in the pharynx of L2 stage animals at the indicated developmental stages. lin-29b is expressed in the pharynx throughout larval and adult development. Scale bars: 10 μm. (C) Confocal images of endogenously tagged LIN-29 protein isoforms in the tail region at the indicated developmental stages. LIN-29b first accumulates in the two rectal cells B and P12.pa, before accumulating in the four additional rectal cells F, K.a, K’ and U (the latter two are not visible in this focal plane). LIN-29a is not detected in these cells. Scale bars: 10 μm.