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Schematic representation of TNAP incorporation and orientation into DPPC:CHOL monolayers. The higher “fluidity” (compressibility) of DPPC:CHOL (4:1) compared to DPPC:CHOL (1:1) monolayer favors TNAP penetration but it hampers TNAP transfer to solid supports and decreases the catalytic activity of incorporated enzymes.
