Table 1:

Characteristics of the Langmuir troughs. The compression rate was kept constant at 0.44 mm.s⁻¹ for all the experiments. All the TNAP-containing monolayers were obtained on 5 mmol.L⁻¹ Tris-HCl buffer with 150 mmol.L⁻¹ NaCl and 15 mmol.L⁻¹ MgCl₂ (pH 8.5) subphase, at a final enzyme concentration of 0.04 μg.mL⁻¹.

Manufacturer	Experiment	Area (cm2)	Volume (mL)
Insight	π-A isotherms	216	120
PMI550	PM-IRRAS and BAM	225	120
Insight	Fluorescence microscopy	14.5	60