Fig. 4. Knockdown of Bcl-3 decreases hypoxia-induced autophagy. AGS and NCI-N87 cells were transfected with si-NC/si-Bcl-3 with or without Rapa. (A) Wound healing assay was used to analyze the migration of AGS and NCI-N87 cells. (B) Quantification of cell migration in AGS and NCI-N87 cells. (C) NCI-N87 cells were transfected with si-NC/si-Bcl-3 with or without 5-FU/Rapa, MTT was used to detect cell proliferation. (D) Relative expression of autophagy-related proteins in AGS and NCI-N87 were analyzed by western blotting. (E, F) Quantification of the relative protein levels of Atg5 and LC3 in AGS and NCI-N87 cells.

Bcl-3 = B-cell lymphoma 3; si-Bcl-3 = si-RNA-Bcl-3; si-NC = si-negative control; 5-FU = 5-fluorouracil; Rapa = rapamycin; GAPDH = glyceraldehyde 3-phosphate dehydrogenase.

^*P<0.05 (compared to control); ^†P<0.05 (compared to si-Bcl-3); ^‡P<0.05 (compared to si-Bcl-3+Rapa); ^§P<0.05 (compared to 5-FU+Rapa); ^∥P<0.05 (compared to si-Bcl-3+5-FU);); ^¶P<0.05 (compared to si-Bcl-3); ^**P<0.05 (compared to si-Bcl-3+Rapa).