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. 2020 Mar 30;11:1609. doi: 10.1038/s41467-020-15326-5

Fig. 7. CD140b+CD184− enrichment improves suboptimal hPSC-RPE differentiations.

Fig. 7

a Representative bright field pictures of HS980 and HS983a hESC lines and CTRL-7-II and CTRL-14-II hiPSC at day 60 of differentiation after being sorted for CD140b+/CD184− or replated 1:20 at day 30. b Bright field and immunofluorescent images showing pigmentation and BEST-1 expression on cytospin mounts of day 60 hPSC-RPE that were sorted for CD140b+/CD184− or replated 1:20 at day 30. Bar graphs compares the percentage of cells that are BEST-1+, manually quantified from four replicated cytospin mounts between sorted and replated hPSC-RPE in each of the four lines tested. Bars represent means ± SEM from four different images. c Functional assays demonstrating monolayer integrity measured by transepithelial resistance (TEER), and pigment epithelium-derived factor (PEDF) polarized secretion measured by ELISA after 60 days of differentiation in the four hPSC-RPE lines sorted for CD140b+/CD184− or replated 1:20 at day 30. The TEER value for undifferentiated hESC is shown for comparison (dashed line). Bars represent means ± SEM from six (TEER) and three (PEDF secretion) independent experiments. (*) Asterisks represent significance with a P value = 0.0015 (b, HS980); =0.0042 (b HS983a); =0.046 (c, TEER, HS980); <0.0001 (c TEER, CTRL-7-II, and CTRL-14-II); <0.0001 (c TEER, CTRL-14-II). Scale bars: a = 100 μm; b = 50 μm. Source data are provided as a Source Data file.