Figure 4.

Demetallation kinetics of Cu•HSA by His and determination of dissociation constant for Cu•HSA. Decrease of relative content of Cu-63 in HSA in time (a); relative content of Cu-63 in HSA at equilibrium at different concentrations of His (b). Conditions: 10 μM Cu•HSA, 10–2000 μM His; incubation buffer 50 mM HEPES, 50 mM NaCl, pH 7.4; LC-ICP MS: column - 1 ml Sephadex G25 Superfine; elution buffer 200 mM NH₄NO₃, pH 7.4; flow rate 0.4 ml/min; injection volume 10 μl, Cu-63 was monitored by ICP MS. Figure was created and fittings were performed by program Origin 9 Pro (https://www.originlab.com/).