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. 2020 Mar 30;10:5682. doi: 10.1038/s41598-020-62507-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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qPCR validation of candidate genes in domestic and wild silkworms. (a) Comparison of cocoon of the domestic strain Dazao (D_DZ), Xiafang (D_XF) and wild silkworm. (b) Whole cocoon weight of the wild silkworm and domestic strains used for qPCR analysis. (c,d) qPCR validation of the interesting candidate genes in the silk gland on day 2 of the wild and domestic silkworms. The design of the specific primers and the qPCR strategy were similar to our previous study³⁸. The primer sequences are listed in Supplementary Table S7. One-way analysis of variance (ANOVA) was used to determine significant differences between any two samples. *P < 0.05; **P < 0.01; n.s.: not significant.