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. 2020 Mar 31;6:17. doi: 10.1038/s41421-020-0150-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Schematic representation summarizing the REF6−DNA interactions. The sequence of the dsDNA (NAC004 fragment) used for crystallization is shown with two complementary strands. The residues involved in the interaction with the dsDNA are labeled in magenta, red, and yellow for domains ZnF2, 3, and 4, respectively. The green and black dashed arrows indicate hydrophobic and hydrophilic interactions, respectively. b Close-up view of the interaction between key residues and DNA bases. c EMSA of different REF6 mutants with the dsDNA. d Measurement of the binding affinity of the WT and mutant REF6 with dsDNA by MST. The experiments were repeated for three times.