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. 2020 Mar 31;6:17. doi: 10.1038/s41421-020-0150-6

Fig. 5. Binding of REF6 to different dsDNA fragments.

Fig. 5

a EMSA of REF6 with single-base mutations of the NAC004 fragment. b MST measurement of the binding affinity of m12 (mutated G:C base pair with an A:T base pair) with REF6. c Modeling of the m12 and REF6 interaction. The mutation from C9′ (left) to T9′ (right) creates a steric clash with REF6. REF6 can bind to both CUC2 (d) and CUC1 (e) dsDNA fragments based on EMSA. f Comparison of the binding affinities of CUC1 and CUC2 with REF6. Note that CUC1-3 + 4 has two CTCTGYTY motifs.