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. 2020 Mar 30;10:5681. doi: 10.1038/s41598-020-62541-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental protocol and anthropometric measurements. (A) Experimental protocol used in this study. C57BL/6 mice (BL) and KK-A^y mice (KK) were fed a normal (N) or high fat diet (H), and were grouped as BL_N, BL_H, KK_N, and KK_H, respectively. Blood (Δ) and feces (▲) were collected at the indicated timepoints. Body weight (B) and the amount of food intake (C) were evaluated every week. Levels of plasma glucose (D) and TG (E) were determined every 4 weeks.