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. 2020 Mar 30;10:5681. doi: 10.1038/s41598-020-62541-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pathophysiological change in mice liver and feces. (A) HE staining and IHC of liver and WAT. Mouse livers and WAT collected at week 20 were prepared and stained with HE and IHC for F4/80. Representative images (scale bar = 100 µm) are shown. (B) Inflammatory gene expressions in mouse livers at week 20. RT-qPCR (n = 5–6 in each group) were performed as described in the Materials and Method. *p < 0.05, **p < 0.01 compared with the BL_N group by one-way ANOVA with post hoc test (Bonferroni). (C) Microbial composition in mouse feces collected at weeks 1, 9 and 17. 16 s rRNA sequencing was performed. Relative abundance and taxonomic classification at the phylum level were analyzed by QIIME. (D) PCA of microbial composition in mouse feces. Data were analyzed by QIIME.