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. 2020 Mar 30;10:5681. doi: 10.1038/s41598-020-62541-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fecal VOC profile at weeks 5 (A), 9 (B), 13 (C), and 17 (D). Feces collected from C57BL/6 and KK-A^y mice fed with a normal or high fat diet were subjected to HSS-GC-MS, then the integrated peak area was used as a variable in PCA. Results show the score plot (left), loading plot (center), and a diagram (right) summarizing the significance (p < 0.05) by two-way ANOVA with multiple testing correction using the Bonferroni family-wise error rate. Ellipses drawn on PCA score plot do not reflect any statistical significance.