Table 2.

Methylation types of Hpa II and Hae II restriction enzymes according to their digestion abilities

Type	Methylation pattern		Restriction state		Score of band profile
	Hpa II	Hae II	Hpa II	Hae II	x	y	z
I Non-methylation	CCGGGGCC	GCGCCGCG	Digested	Digested	−/1	+/0	+/0
II Semi-methylation	CCGGGGCC	GCGCCGCG	Digested	Undigested	−/1	+/0	−/1
III Full-methylation	CCGGGGCC	GCGCCGCG	Undigested	Digested	−/1	−/1	+/0
IV Full-methylation	CCGGGGCC/CCGG GGCC	GCGCCGCG/GCGCCGCG	Undigested	Undigested	−/1	−/1	−/1

Score of band profile in the table indicates if PCR product is not digested by either enzyme (x), digested by Hpa II (y) and digested by Hae II (z). Scores “+” refer to digestion and “−” refer to undigestion while “1” refer to band presence and “0” refer to band absence