Table 1.
Purification of cellobiohydrolases (CBH) from Schizophyllum commune KMJ820
| Step | Total protein (mg) | Specific activity (U/mg) | Total activity (U/ml) | Yield (%) | Purification fold | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| CBH 1 | CBH 2 | CBH 1 | CBH 2 | CBH 1 | CBH 2 | CBH 1 | CBH 2 | CBH 1 | CBH 2 | |
| Crude extract | 463.4 | 463.4 | 11.51 | 11.51 | 5335 | 5335 | 100 | 100 | – | – |
| Ammonium sulfate precipitation | 162.9 | 162.9 | 12.84 | 12.84 | 2092 | 2092 | 39.22 | 39.22 | 1.12 | 1.12 |
| DEAE chromatography-1 | 33.30 | 3.00 | 19.57 | 18.12 | 651.8 | 54.32 | 12.22 | 1.02 | 1.70 | 1.57 |
| Hydroxyapatite | 2.66 | – | 21.37 | – | 56.82 | – | 1.07 | – | 1.86 | – |
| DEAE chromatography-2 | 0.22 | – | 37.63 | – | 8.34 | – | 0.16 | – | 3.27 | – |