Fig. 3.

NVs and FT/NVs suppressed TNF-α-induced inflammation in HCAECs. HCAECs were pretreated with NV or FT/NV for 2 h and then stimulated with TNF-α (10 ng/mL) for 6 h. A Calcein-AM-labeled THP-1 cells were incubated with stimulated-HCAECs for 1 h. Adherent THP-1 cells on HCAECs were detected by a fluorescence microscope (× 40, scale bars = 200 μm). B Adherent THP-1 cells were counted and quantified. The C mRNA and D protein expression levels of VCAM-1 were analyzed by real-time qPCR and Western blot, respectively. Data are mean ± SD from 3 independent experiments. ***p < 0.001