Table 1.
Primer sets designed from selected sequences of M. kansasii subtypes I-V.
| Primer ID | Target gene | Amplicon Length (bp) | Forward Primer (5′-3′) | Reverse Primer (5′-3′) |
|---|---|---|---|---|
| DPT1a | kaust1_03087 | 199 | GTTCGTCTCGATTTCGCAGC | GAATCACGCGCCTTGATGAC |
| DPT2 | kaust2_05863 | 312 | TTTCGGACAATGACGGCGGACG | ATGCAGTGTCCGGCAAAGGGGT |
| DPT3 | kaust3_04009 | 250 | TGGCGGGTGTGTTGATGATGGC | ATCAGCGGCAACGGCGGTAA |
| DPT4 | kaust4_00078 | 111 | GATGGTCAAATCGAGCGACGAGGCG | GCGACGGGTTCATCGGCAGTGATT |
| DPT5 | kaust5_02691 | 445 | ACGCCTTGGAACGTGACCGTGA | ACTGATTCGTGGCCCGGATGGA |
| Internal control kaust1_00615, kaust2_00230, kaust3_03982, kaust4_05573, kaust5_02753b | 591 | TGGCGCATGCCTTGTGGTGGAA | TCCTGTTCGCTCCCCACGCTTT | |
a
Diagnostic primer for subtype I.
b
Internal control that targets the 16S rRNA gene.