FIGURE 3.

Construction and characterization of the bio-oxygen pump in vitro. (A) Scheme of oxygen generation system based on S. 2973 under 640 nm red laser irradiation. (B) Absorption spectrum and OD_{750 nm} of S. 2973 irradiated using 640 nm laser with different intensities. (C) Absorption spectrum and OD_{750 nm} of S. 2973 irradiated using 640 nm laser with 0.25 W/cm² for different times. (D) Oxygen evolution of S. 2973 under 640 nm red laser irradiation. (E) Cell viability and AM/PI staining of 4T1 cells with or without treatments (i.e., 640 nm laser irradiation, co-cultivation with S. 2973 or both of them) for 24 h. (F) Phenotypes of S. 2973 treated with different concentrations of ICG exposed to 2 W/cm² of 808 nm laser for 5 min.